Nature Communications (Aug 2022)
Omicron SARS-CoV-2 mutations stabilize spike up-RBD conformation and lead to a non-RBM-binding monoclonal antibody escape
- Zhennan Zhao,
- Jingya Zhou,
- Mingxiong Tian,
- Min Huang,
- Sheng Liu,
- Yufeng Xie,
- Pu Han,
- Chongzhi Bai,
- Pengcheng Han,
- Anqi Zheng,
- Lutang Fu,
- Yuanzhu Gao,
- Qi Peng,
- Ying Li,
- Yan Chai,
- Zengyuan Zhang,
- Xin Zhao,
- Hao Song,
- Jianxun Qi,
- Qihui Wang,
- Peiyi Wang,
- George F. Gao
Affiliations
- Zhennan Zhao
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Jingya Zhou
- University of Chinese Academy of Sciences
- Mingxiong Tian
- College of life Science, Shanxi University
- Min Huang
- School of Life Science, University of Science and Technology of China
- Sheng Liu
- Cryo-EM Center, Department of Biology, Southern University of Science and Technology
- Yufeng Xie
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Pu Han
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Chongzhi Bai
- Central Laboratory, Shanxi Province Hospital of Traditional Chinese Medicine
- Pengcheng Han
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Anqi Zheng
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Lutang Fu
- Cryo-EM Center, Department of Biology, Southern University of Science and Technology
- Yuanzhu Gao
- Cryo-EM Center, Department of Biology, Southern University of Science and Technology
- Qi Peng
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Ying Li
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Yan Chai
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Zengyuan Zhang
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Xin Zhao
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Hao Song
- Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences
- Jianxun Qi
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Qihui Wang
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Peiyi Wang
- Cryo-EM Center, Department of Biology, Southern University of Science and Technology
- George F. Gao
- CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- DOI
- https://doi.org/10.1038/s41467-022-32665-7
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 12
Abstract
The SARS-CoV-2 Omicron variant spreads rapidly. Here the authors show that Omicron S preferentially adopts the one-RBD-up conformation, which leads to a non-RBM-binding monoclonal antibody escape. Mutagenesis reveals that S371L, S373P and S375F substitutions enhance the conformational stability.
