Detection of Cryptosporidium parvum from feces samples of human and camels by using direct Polymerase Chain Reaction assay technique

Abstract

The study was designed for molecular detected of Cryptosporidium parvum from human and camel by using direct Polymerase Chain Reaction assay technique. A total of 50 fecal samples from human and 50 samples from camel collected. The Cryptosporidium parvum positive isolates were identified by using specific primers for heat shock protein gene that designed in this study using NCBI-Genbank database (Genbank code: GQ259151.1) and primer3 plus program for primer design. Results show that prevalence of infection with Cryptosporidium parvum was (24%) 12 positive out of 50 human fecal samples, whereas the prevalence of infection with Cryptosporidium parvum in camel was (14%) 7 positive out of 50 fecal samples. The study demonstrates that the direct Polymerase Chain Reaction (PCR) assay technique is a simple, rapid and valuable tool for the detection of Cryptosporidium parvum.