The impairment of osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells under high D-glucose concentrations

Abstract

Type 2 diabetic patients have an increased risk of developing serious long-term complications such as cardiovascular disease, retinopathy, nephropathy, neuropathy, diabetic foot, and osteoporosis. However, the correlation between hyperglycaemia and osteoporosis has not yet been fully clarified. In this research, we investigated the effect of different high D-glucose concentrations (25, 50, and 100 mM) on osteogenic differentiation of human non-diabetic and diabetic adipose tissue-derived mesenchymal stem cells (nAT-MSCs and dAT-MSCs). The differentiated cells were qualified by Alizarin Red S staining and quantified by measuring the absorbance at 482 nm. The expressions of osteogenic master genes were examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) methods. Interestingly, the osteogenic differentiation and the expression of osteogenic-specific genes (Runx-2 and ALP) decreased with an increase of D-glucose concentration. Our work contributes to the understanding of the relationship between hyperglycaemia and osteoporosis and support the role of stem cells in developing new medicine or therapeutic treatment for preventing diabetic complications.

Keywords

• AT-MSCs
• diabetes
• differentiation potential
• high D-glucose concentrations
• osteoporosis