Фармация и фармакология (Пятигорск) (Aug 2023)

Development of composition and technology for obtaining antimicrobial composition based on mono- and sesquiterpenoids

  • E. V. Lakomkina,
  • G. A. Atazhanova,
  • S. B. Akhmetova,
  • I. N. Zilfikarov

DOI
https://doi.org/10.19163/2307-9266-2023-11-2-114-126
Journal volume & issue
Vol. 11, no. 2
pp. 114 – 126

Abstract

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The article reflects the results of the essential oils selection for the compositions with antibacterial and antifungal properties. The technology of their production is represented.The aim of the work was the development of technology and the study structure of new essential oils compositions with antibacterial and antifungal activities.Material and methods. The following plants have been used in the work: the herb of Hyssopus ambiguus (Trautv.) Iljin, the herb of Thymus crebrifolius Klokov, the herb of Thymus marschallianus Willd, the herb of Thymus serpyllum L., and the essential oils obtained from them. The composition of the essential oils was determined by Gas Chromatography Mass Spectrometry. The main physical and chemical parameters of the compositions were evaluated in accordance with the requirements of the Russian State Pharmacopoeia, the XIVth edition. The tests for the antimicrobial activity were carried out using the strains of Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Candida albicans ATCC 10231.Results. Compositions with an activity against microorganisms Staphylococcus aureus, Bacillus subtilis, Escherichia coli and fungi Candida albicans have been obtained and studied. The composition based on essential oils of Hyssopus ambiguus (Trautv.) Iljin and Thymus marschallianus Willd. contained 139 components, the main ones of which are eucalyptol (6.51%) and terpinen-4-ol (1.95%). The composition of essential oils of Hyssopus ambiguus (Trautv. Iljin) and Thymus crebrifolius Klokov contained 137 components (eucalyptol (20.37%), terpinen-4-ol (7.03%), Ʈ-muurol (2.28%), γ-terpinene (2.23%), β-myrcene (2.09%), etc.). The composition of the essential oils of Hyssopus ambiguus (Trautv.) Iljin and Thymus serpyllum L. contained 149 components (the main ones are eucalyptol (7.33%) and α-terpineol (0.9%)).Conclusion. The technology has been proposed and the structure of the essential oils compositions with antibacterial and antifungal activities has been established.

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