A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging

Yubing Han; Zhimin Zhang; Wenjie Liu; Yuanfa Yao; Yingke Xu; Xu Liu; Xu Liu; Xu Liu; Cuifang Kuang; Cuifang Kuang; Cuifang Kuang; Xiang Hao

doi:10.3389/fchem.2020.601436

Frontiers in Chemistry (Jan 2021)

A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging

Yubing Han,
Zhimin Zhang,
Wenjie Liu,
Yuanfa Yao,
Yingke Xu,
Xu Liu,
Xu Liu,
Xu Liu,
Cuifang Kuang,
Cuifang Kuang,
Cuifang Kuang,
Xiang Hao

Affiliations

Yubing Han: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Zhimin Zhang: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Wenjie Liu: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Yuanfa Yao: Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, China
Yingke Xu: Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, China
Xu Liu: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Xu Liu: Ningbo Research Institute, Zhejiang University, Ningbo, China
Xu Liu: Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, China
Cuifang Kuang: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Cuifang Kuang: Ningbo Research Institute, Zhejiang University, Ningbo, China
Cuifang Kuang: Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, China
Xiang Hao: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China

DOI: https://doi.org/10.3389/fchem.2020.601436
Journal volume & issue: Vol. 8

Abstract

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Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overcome these limits—taking advantage of the intrinsic affinity of bright and photostable fluorophores, no matter if they are supposed to be live-cell incompatible or not. Incubated with living cells and tissues in particular conditions (concentration and temperature), some Atto and BODIPY dyes show live-cell labeling capability for specific organelles without physical cell-penetration or chemical modifications. Notably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability compared with the most commonly used SiR dye in long-term imaging. Our strategy has expanded the scientist's toolbox for understanding the dynamics and interactions of subcellular structures in living specimens.

Published in Frontiers in Chemistry

ISSN: 2296-2646 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: http://journal.frontiersin.org/journal/chemistry

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