Analysis of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection

Zi-You Cai; Yong-Chong Li; Li-Hua Li; Zuan-Guang Chen

Journal of Pharmaceutical Analysis (Oct 2012)

Analysis of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection

Zi-You Cai,
Yong-Chong Li,
Li-Hua Li,
Zuan-Guang Chen

Affiliations

Zi-You Cai: Guangdong Food and Drug Vocational College, Guangzhou 510520, China; Corresponding authors. Tel.: +86 20 3994 3044; fax: +86 20 3994 3071.
Yong-Chong Li: Guangdong Food and Drug Vocational College, Guangzhou 510520, China
Li-Hua Li: Guangdong Food and Drug Vocational College, Guangzhou 510520, China
Zuan-Guang Chen: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China; Corresponding authors. Tel.: +86 20 3994 3044; fax: +86 20 3994 3071.

Journal volume & issue: Vol. 2, no. 5
pp. 356 – 360

Abstract

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A new method for the determination of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection (MCE-CCD) was proposed. The effects of various electrophoretic operating parameters on the analysis of arecoline were studied. Under the optimal conditions, arecoline was rapidly separated and detected in 1Â min with good linearity over the concentration range of 20â1500Â Î¼M (r2=0.9991) and the detection limit of 5Â Î¼M (S/N=3). The method was used for the analysis of arecoline satisfactorily with a recovery of 96.8â104%. Keywords: Microchip capillary electrophoresis, Contactless conductivity detection, Arecoline, Semen Arecae

Published in Journal of Pharmaceutical Analysis

ISSN: 2095-1779 (Print); 2214-0883 (Online)
Publisher: Elsevier
Country of publisher: China
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmaceutical-analysis/

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