A cell-free platform for the prenylation of natural products and application to cannabinoid production

Meaghan A. Valliere; Tyler P. Korman; Nicholas B. Woodall; Gregory A. Khitrov; Robert E. Taylor; David Baker; James U. Bowie

doi:10.1038/s41467-019-08448-y

Nature Communications (Feb 2019)

A cell-free platform for the prenylation of natural products and application to cannabinoid production

Meaghan A. Valliere,
Tyler P. Korman,
Nicholas B. Woodall,
Gregory A. Khitrov,
Robert E. Taylor,
David Baker,
James U. Bowie

Affiliations

Meaghan A. Valliere: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Tyler P. Korman: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Nicholas B. Woodall: Department of Biochemistry, Institute for Protein Design, University of Washington
Gregory A. Khitrov: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Robert E. Taylor: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
David Baker: Department of Biochemistry, Institute for Protein Design, University of Washington
James U. Bowie: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California

DOI: https://doi.org/10.1038/s41467-019-08448-y
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 9

Abstract

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Producing individual cannabinoids by metabolically engineered microbes has proven challenging. Here, the authors develop a cell-free enzymatic prenylating system to generate isoprenyl pyrophosphate substrates directly from glucose and produce both common and rare cannabinoids at >1 g/L.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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