Journal of Veterinary Research (Dec 2018)

Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples

  • Kędrak-Jabłońska Agnieszka,
  • Budniak Sylwia,
  • Szczawińska Anna,
  • Reksa Monika,
  • Krupa Marek,
  • Szulowski Krzysztof

DOI
https://doi.org/10.2478/jvetres-2018-0075
Journal volume & issue
Vol. 62, no. 4
pp. 549 – 554

Abstract

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Introduction: The aim of the study was the application and evaluation of real-time PCRs based on the fluorescence of SYBR Green I intercalating dye for the detection of three Bacillus anthracis genes in contaminated liver and blood samples. The goals for detection were rpoB gene as a chromosomal marker, pag gene located on plasmid pXO1, and capC gene located on plasmid pXO2.

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