Frontiers in Molecular Biosciences (Jan 2024)

Exploration of the hypoglycemic mechanism of Fuzhuan brick tea based on integrating global metabolomics and network pharmacology analysis

  • Xingliang Xiang,
  • Xingliang Xiang,
  • Shanqin You,
  • Zhaoxiang Zeng,
  • Jinlin Xu,
  • Jinlin Xu,
  • Yuqi Lin,
  • Yukun Liu,
  • Lijun Zhang,
  • Rongzeng Huang,
  • Rongzeng Huang,
  • Chengwu Song,
  • Chengwu Song,
  • Shuna Jin,
  • Shuna Jin

DOI
https://doi.org/10.3389/fmolb.2023.1266156
Journal volume & issue
Vol. 10

Abstract

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Introduction: Fuzhuan brick tea (FBT) is a worldwide popular beverage which has the appreciable potential in regulating glycometabolism. However, the reports on the hypoglycemic mechanism of FBT remain limited.Methods: In this study, the hypoglycemic effect of FBT was evaluated in a pharmacological experiment based on Kunming mice. Global metabolomics and network pharmacology were combined to discover the potential target metabolites and genes. In addition, the real-time quantitative polymerase chain reaction (RT-qPCR) analysis was performed for verification.Results: Seven potential target metabolites and six potential target genes were screened using the integrated approach. After RT-qPCR analysis, it was found that the mRNA expression of VEGFA, KDR, MAPK14, and PPARA showed significant differences between normal and diabetes mellitus mice, with a retracement after FBT treatment.Conclusion: These results indicated that the hypoglycemic effect of FBT was associated with its anti-inflammatory activities and regulation of lipid metabolism disorders. The exploration of the hypoglycemic mechanism of FBT would be meaningful for its further application and development.

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