CyTOF protocol for immune monitoring of solid tumors from mouse models

Sarah M. Shin; Alexei Hernandez; Erin Coyne; Kabeer Munjal; Nicole E. Gross; Soren Charmsaz; Xuan Yuan; Hongqui Yang; Won Jin Ho

STAR Protocols (Mar 2023)

CyTOF protocol for immune monitoring of solid tumors from mouse models

Sarah M. Shin,
Alexei Hernandez,
Erin Coyne,
Kabeer Munjal,
Nicole E. Gross,
Soren Charmsaz,
Xuan Yuan,
Hongqui Yang,
Won Jin Ho

Affiliations

Sarah M. Shin: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
Alexei Hernandez: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
Erin Coyne: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
Kabeer Munjal: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
Nicole E. Gross: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
Soren Charmsaz: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA
Xuan Yuan: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA; Mass Cytometry Facility at Johns Hopkins University, Baltimore, MD 21287, USA
Hongqui Yang: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA; Mass Cytometry Facility at Johns Hopkins University, Baltimore, MD 21287, USA
Won Jin Ho: Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA; Mass Cytometry Facility at Johns Hopkins University, Baltimore, MD 21287, USA; Convergence Institute, Johns Hopkins University, Baltimore, MD 21287, USA; Corresponding author

Journal volume & issue: Vol. 4, no. 1
p. 101949

Abstract

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Summary: Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects.For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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