Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in <i>Xenopus tropicalis snai2:eGFP</i> line

Jian Wang; Congyu Lu; Shuo Wei

doi:10.1093/g3journal/jkac037

G3: Genes, Genomes, Genetics (Feb 2022)

Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in <i>Xenopus tropicalis snai2:eGFP</i> line

Jian Wang,
Congyu Lu,
Shuo Wei

Affiliations

Jian Wang: ORCiD; Department of Biological Sciences and Center for Bioinformatics and Computational Biology, University of Delaware, Newark, DE 19716, USA
Congyu Lu: ORCiD; Department of Biological Sciences and Center for Bioinformatics and Computational Biology, University of Delaware, Newark, DE 19716, USA
Shuo Wei: ORCiD; Department of Biological Sciences and Center for Bioinformatics and Computational Biology, University of Delaware, Newark, DE 19716, USA

DOI: https://doi.org/10.1093/g3journal/jkac037
Journal volume & issue: Vol. 12, no. 5

Abstract

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AbstractTransgenesis with the meganuclease I-SceI is a safe and efficient method, but the underlying mechanisms remain unclear due to the lack of information on transgene localization. Using I-SceI, we previously developed a transgenic Xenopus tropicalissnai2snai2:eGFPXenopus tropicalis

Published in G3: Genes, Genomes, Genetics

ISSN: 2160-1836 (Online)
Publisher: Oxford University Press
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Genetics
Website: https://academic.oup.com/g3journal

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