Viruses (Dec 2022)

Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves

  • Alexandra Buckley,
  • Lauren Crawford,
  • Kyle Hoffman,
  • Shollie Falkenberg

DOI
https://doi.org/10.3390/v14122809
Journal volume & issue
Vol. 14, no. 12
p. 2809

Abstract

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Senecavirus A (SVA) is a causative agent for vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases of swine including foot-and-mouth disease (FMD). Recently, it was reported that buffalo in Guangdong, China were experiencing clinical symptoms similar to FMD including mouth ulcers and lameness tested positive for SVA. The objective of this study was to determine the susceptibility of cattle (Bos taurus) to SVA infection. Initial in vitro work using the PrimeFlow assay demonstrated that bovine cell lines and peripheral blood mononuclear cells from cattle were susceptible to SVA infection. Subsequently, six colostrum-deprived Holstein calves were challenged with SVA intranasally. No vesicular lesions were observed after challenge. Serum, oral, nasal, and rectal swabs tested for SVA nucleic acid did not support significant viral replication and there was no evidence of seroconversion. Therefore, demonstrating cattle from this study were not susceptible to experimental SVA infection.

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