Whole-genome analysis of coxsackievirus B5 isolated in Yunnan province, China in 2022

Zhaoyang CHU; Junwei CHEN; Changzeng FENG; Yuhan LIU; Ming ZHANG; Li LI; Shaohui MA

doi:10.11847/zgggws1144519

Zhongguo gonggong weisheng (Sep 2024)

Whole-genome analysis of coxsackievirus B5 isolated in Yunnan province, China in 2022

Zhaoyang CHU,
Junwei CHEN,
Changzeng FENG,
Yuhan LIU,
Ming ZHANG,
Li LI,
Shaohui MA

Affiliations

Zhaoyang CHU: Key Laboratory of Vaccine Research and Development for Major Infectious Diseases in Yunnan Province, Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China
Junwei CHEN: Key Laboratory of Vaccine Research and Development for Major Infectious Diseases in Yunnan Province, Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China
Changzeng FENG: Key Laboratory of Vaccine Research and Development for Major Infectious Diseases in Yunnan Province, Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China
Yuhan LIU: Key Laboratory of Vaccine Research and Development for Major Infectious Diseases in Yunnan Province, Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China
Ming ZHANG: Key Laboratory of Vaccine Research and Development for Major Infectious Diseases in Yunnan Province, Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China
Li LI: Department of Clinical Laboratory, Kunming Maternal and Child Health Care Hospital, Kunming 650031, China
Shaohui MA: Key Laboratory of Vaccine Research and Development for Major Infectious Diseases in Yunnan Province, Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China

DOI: https://doi.org/10.11847/zgggws1144519
Journal volume & issue: Vol. 40, no. 9
pp. 1033 – 1039

Abstract

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ObjectiveTo analyze the whole-genome characteristics of four coxsackievirus B5 (CVB5) strains isolated in Yunnan province, China in 2022. MethodsPrimers were designed to amplify and sequence the whole genome of CVB5 isolates. The whole genome was amplified in segments, and sequenced using the "primer walking" method. Sequence splicing and assembly were performed. Genetic characteristics of the CVB5 strains were analyzed using software such as MEGA 7.0, Simplot 3.5.1, and DNAStar 7.1. ResultsBased on VP1 and whole-genome phylogenetic analysis, all four CVB5 isolates belonged to genotype C. Compared with the prototype strain, multiple amino acid mutations occurred in the VP1 region of the four strains. Phylogenetic analysis of P1, P2, and P3 regions and Simplot recombination analysis showed that the CVB5 isolates in this study may have undergone recombination with multiple enterovirus B strains. ConclusionThe four CVB5 strains isolated from clinical samples of hand, foot and mouth disease (HFMD) patients in Kunming, Yunnan province in 2022 all belonged to genotype C.

Published in Zhongguo gonggong weisheng

ISSN: 1001-0580 (Print)
Publisher: Editorial Office of Chinese Journal of Public Health
Country of publisher: China
LCC subjects: Medicine: Public aspects of medicine
Website: https://www.zgggws.com/indexen.htm

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