Communications Biology (May 2022)
Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis
- Hajime Shinoda,
- Tatsuya Iida,
- Asami Makino,
- Mami Yoshimura,
- Junichiro Ishikawa,
- Jun Ando,
- Kazue Murai,
- Katsumi Sugiyama,
- Yukiko Muramoto,
- Masahiro Nakano,
- Kotaro Kiga,
- Longzhu Cui,
- Osamu Nureki,
- Hiroaki Takeuchi,
- Takeshi Noda,
- Hiroshi Nishimasu,
- Rikiya Watanabe
Affiliations
- Hajime Shinoda
- Molecular Physiology Laboratory, Cluster for Pioneering Research
- Tatsuya Iida
- Molecular Physiology Laboratory, Cluster for Pioneering Research
- Asami Makino
- Molecular Physiology Laboratory, Cluster for Pioneering Research
- Mami Yoshimura
- Molecular Physiology Laboratory, Cluster for Pioneering Research
- Junichiro Ishikawa
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
- Jun Ando
- Molecular Physiology Laboratory, Cluster for Pioneering Research
- Kazue Murai
- Molecular Physiology Laboratory, Cluster for Pioneering Research
- Katsumi Sugiyama
- FUJIFILM Media Crest Co., Ltd
- Yukiko Muramoto
- CREST, Japan Science and Technology Agency
- Masahiro Nakano
- CREST, Japan Science and Technology Agency
- Kotaro Kiga
- Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University
- Longzhu Cui
- Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University
- Osamu Nureki
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
- Hiroaki Takeuchi
- Department of Molecular Virology, Tokyo Medical and Dental University
- Takeshi Noda
- CREST, Japan Science and Technology Agency
- Hiroshi Nishimasu
- CREST, Japan Science and Technology Agency
- Rikiya Watanabe
- Molecular Physiology Laboratory, Cluster for Pioneering Research
- DOI
- https://doi.org/10.1038/s42003-022-03433-6
- Journal volume & issue
-
Vol. 5,
no. 1
pp. 1 – 8
Abstract
An automated amplification-free digital RNA detection platform using CRISPR-Cas13a and magnetic bead technology implemented in a microchamber device demonstrates sensitive SARS-CoV-2 genomic RNA detection and variant discrimination.
