Determination of the Suitable Indices for the Evaluation of Drought Tolerance in Two Species of Orchardgrass (Dactylis glomerata) and Smooth Bromegrass (Bromus inermis)

Abstract

Drought is one of the most important abiotic stresses limiting the survival, growth, and productivity of plants in many regions of the world. This study was conducted to evaluate drought tolerance of the recombinant genotypes of two species of orchardgrass and smooth bromegrass based on clonal evaluation and stress tolerance indices. In this study, 36 genotypes selected from polycross populations of both species of orchardgrass and smooth bromegrass were evaluated in terms of phenological, agronomic and morphological traits under normal (50% water depletion from the root zone depth) and drought-stressed (90% water depletion from the root zone depth) conditions during 2013 to 2015 at the Research Farm of Isfahan University of Technology, Isfahan, Iran. To evaluate drought tolerance of the mentioned genotypes, five drought tolerance and susceptibility indices including stress tolerance indices (TOL and STI), mean productivity index (MP), geometric mean productivity (GMP) and stress sensitivity index (SSI) were calculated based on forage yield under drought stress (Ys) and non- stress (Yp) conditions. In both species, high genetic variation was observed among genotypes in terms of most of the studied traits and indices. In orchardgrass, the genetic coefficient of variation varied from 4.31% to 23.5% under normal condition, and from 1.31% to 21.3% under drought stress condition. In smooth bromegrass, the range of genetic coefficient of variation was variable from 2.05-21.4% under normal condition and 1.66-24.6% under drought stress condition. This indicated a high potential for improving these traits through targeted selection in breeding programs of the two species. Drought stress significantly affected most of the studied traits and reduced the genetic variability of traits except for days to ear emergence, days to pollination, percentage of dry matter yield of cut 1, and percentage of dry matter yield of cut 2 in orchardgrass, and percentage of dry matter yield of cut 1 and percentage of dry matter yield of cut 3 in smooth bromegrass. The amount of this reduction for dry matter yield of cuts 1, 2 and 3 was 47.5, 67.3, and 66.6 percent, respectively, in orchardgrass, and 37.4, 49.5, and 49.4 percent, respectively, in smooth bromegrass. Low heritability for forage yield (24.7, 13.5, and 25.9 percent for dry matter yield of cut 1, dry matter yield of cut 2, and dry matter yield of cut 3, respectively, in orchardgrass, and 59.5, 45.9, and 59.4 percent for dry matter yield of cut 1, dry matter yield of cut 2, and dry matter yield of cut 3, respectively, in smooth bromegrass) suggested that direct selection based on forage yield is not effective for the improvement of this trait. Though, indirect selection based on components of forage yield which have high heritability and high correlation with yield (i.e. plant height, stems/plant, and crown diameter in both species), would be more effective. In both species, selection indices of MP, GMP, and STI had a positive and significant correlation with forage yield (0.92, 0.75, and 0.75, respectively, under normal condition, and 0.61, 0.81, and 0.77, respectively, under drought-stressed condition, in orchardgrass; and 0.95, 0.92, and 0.88, respectively, under normal condition, and 0.91, 0.95, and 0.95, respectivel, under drought-stressed condition, in smooth bromegrass). Therefore, these indices were identified as desirable ones in the selection of drought tolerant cultivars in orchardgrass and smooth bromegrass. Based on the results of principal component analysis and drought tolerance and susceptibility indices, the genotypes of 13 and 14 in orchardgrass, and 13 and 32 in smooth bromegrass were identified as superior genotypes in terms of forage yield and drought tolerance. These genotypes can be used as superior parents for the development of genetic populations to improve forage yield and stress tolerance and create synthetic cultivars.

Keywords

• bromus
• moisture stress
• dactylis
• stress tolerance and susceptibility indices