TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells

Jiajing Chen; Zikang Wang; Xudong Guo; Fudong Li; Qingtao Wei; Xuwen Chen; Deshun Gong; Yanxin Xu; Wen Chen; Yongrui Liu; Jiuhong Kang; Yunyu Shi

doi:10.1038/s41467-019-12126-4

Nature Communications (Sep 2019)

TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells

Jiajing Chen,
Zikang Wang,
Xudong Guo,
Fudong Li,
Qingtao Wei,
Xuwen Chen,
Deshun Gong,
Yanxin Xu,
Wen Chen,
Yongrui Liu,
Jiuhong Kang,
Yunyu Shi

Affiliations

Jiajing Chen: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China
Zikang Wang: Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Institute for Advanced Study, Tongji University
Xudong Guo: Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Institute for Advanced Study, Tongji University
Fudong Li: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China
Qingtao Wei: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China
Xuwen Chen: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China
Deshun Gong: Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University
Yanxin Xu: Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Institute for Advanced Study, Tongji University
Wen Chen: Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Institute for Advanced Study, Tongji University
Yongrui Liu: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China
Jiuhong Kang: Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Institute for Advanced Study, Tongji University
Yunyu Shi: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China

DOI: https://doi.org/10.1038/s41467-019-12126-4
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 17

Abstract

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TRIM66 protein has an N-terminal tripartite motif and a C-terminal PHD Bromodomain. Here the authors show the specific histone modification recognition of TRIM66-PHD-Bromodomain through crystallography and biochemistry assay, and further reveal that TRIM66 recognition of certain histone modification is important for DNA damage repair in ESCs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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