Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from <i>Penicillium</i> sp. OUCMDZ-1435

Xingxing He; Yanzheng Jin; Fei Kong; Liyuan Yang; Mingzhuang Zhu; Yi Wang

doi:10.3390/molecules28073180

Molecules (Apr 2023)

Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from <i>Penicillium</i> sp. OUCMDZ-1435

Xingxing He,
Yanzheng Jin,
Fei Kong,
Liyuan Yang,
Mingzhuang Zhu,
Yi Wang

Affiliations

Xingxing He: School of Medicine and Pharmacy, Ocean University of China, Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China
Yanzheng Jin: School of Medicine and Pharmacy, Ocean University of China, Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China
Fei Kong: School of Medicine and Pharmacy, Ocean University of China, Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China
Liyuan Yang: School of Medicine and Pharmacy, Ocean University of China, Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China
Mingzhuang Zhu: Fisheris College, Ocean University of China, Qingdao 266003, China
Yi Wang: School of Medicine and Pharmacy, Ocean University of China, Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China

DOI: https://doi.org/10.3390/molecules28073180
Journal volume & issue: Vol. 28, no. 7
p. 3180

Abstract

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Meleagrin and oxaline, which belong to the roquefortine alkaloids with a unique dihydroindole spiroamide framework, have significant bioactivities, especially tumor cell inhibitory activity. In order to discover the requefortine alkaloids, Penicillium sp. OUCMDZ-1435 was fished and identified from marine fungi using molecular probe technology. Meleagrin (1) and oxaline (2) were isolated from it. In addition, we first reported that compounds 1 and 2 could effectively inhibit the proliferation and metastasis of the human HepG2 cell and induce HepG2 cell apoptosis and cell cycle arrest in the G2/M phase. Additionally, the fermentation of Meleagrin (1) was optimized to increase its yield to 335 mg/L. These results provided bioactive inspiration and fungus resources for roquefortine alkaloid development.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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