Isolation of active metabolites of B. ginsengihumi M2.11 with fungicidal activity

Abstract

The purpose of this work is to purify and identify compounds from Bacillus ginsengihumi M2.11 that have fungicidal activity. Isolation of active metabolites was carried out by sample preparation, concentration, and chromatography. Substances were identified using a Maxsis impact mass spectrometer (Bruker). The results obtained were processed in the DataAnalysis Version 4.1 program. We searched library databases presented on the servers http://www.massbank.jp; http://www.chemspider.com. The most likely substance with fungicidal activity is a compound with m/z 1079, which is a lipopeptide. In this work, the fungicidal compound produced by the strain B. ginsengihumi M2.11 is a biosurfactant – disodium surfactin, with molecular formula C53H91N7Na2O13. Thus, the biocontrol function of the B. ginsengihumi M2.11 strain is largely determined by the secretion of surfactants – biosurfactants. Studying the mechanisms and modes of action of the active compound will allow full use in agriculture to protect plants from phytopogens, increase the quality and quantity of yield.