Frontiers in Cell and Developmental Biology (May 2023)
Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models
- Hilansi Rawat,
- Hilansi Rawat,
- Hilansi Rawat,
- Jessica Kornherr,
- Jessica Kornherr,
- Jessica Kornherr,
- Dorota Zawada,
- Dorota Zawada,
- Dorota Zawada,
- Sara Bakhshiyeva,
- Sara Bakhshiyeva,
- Sara Bakhshiyeva,
- Christian Kupatt,
- Karl-Ludwig Laugwitz,
- Andrea Bähr,
- Tatjana Dorn,
- Tatjana Dorn,
- Tatjana Dorn,
- Alessandra Moretti,
- Alessandra Moretti,
- Alessandra Moretti,
- Alessandra Moretti,
- Monika Nowak-Imialek,
- Monika Nowak-Imialek,
- Monika Nowak-Imialek
Affiliations
- Hilansi Rawat
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Hilansi Rawat
- German Center for Cardiovascular Research (DZHK), Munich Heart Alliance, Munich, Germany
- Hilansi Rawat
- Regenerative Medicine in Cardiovascular Diseases, First Department of Medicine, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Jessica Kornherr
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Jessica Kornherr
- German Center for Cardiovascular Research (DZHK), Munich Heart Alliance, Munich, Germany
- Jessica Kornherr
- Regenerative Medicine in Cardiovascular Diseases, First Department of Medicine, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Dorota Zawada
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Dorota Zawada
- German Center for Cardiovascular Research (DZHK), Munich Heart Alliance, Munich, Germany
- Dorota Zawada
- Regenerative Medicine in Cardiovascular Diseases, First Department of Medicine, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Sara Bakhshiyeva
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Sara Bakhshiyeva
- German Center for Cardiovascular Research (DZHK), Munich Heart Alliance, Munich, Germany
- Sara Bakhshiyeva
- Regenerative Medicine in Cardiovascular Diseases, First Department of Medicine, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Christian Kupatt
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Karl-Ludwig Laugwitz
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Andrea Bähr
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Tatjana Dorn
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Tatjana Dorn
- German Center for Cardiovascular Research (DZHK), Munich Heart Alliance, Munich, Germany
- Tatjana Dorn
- Regenerative Medicine in Cardiovascular Diseases, First Department of Medicine, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Alessandra Moretti
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Alessandra Moretti
- German Center for Cardiovascular Research (DZHK), Munich Heart Alliance, Munich, Germany
- Alessandra Moretti
- Regenerative Medicine in Cardiovascular Diseases, First Department of Medicine, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Alessandra Moretti
- Department of Surgery, Yale University School of Medicine, New Haven, CT, United States
- Monika Nowak-Imialek
- First Department of Medicine, Cardiology, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- Monika Nowak-Imialek
- German Center for Cardiovascular Research (DZHK), Munich Heart Alliance, Munich, Germany
- Monika Nowak-Imialek
- Regenerative Medicine in Cardiovascular Diseases, First Department of Medicine, Klinikum Rechts der Isar, School of Medicine and Health, Technical University of Munich, Munich, Germany
- DOI
- https://doi.org/10.3389/fcell.2023.1111684
- Journal volume & issue
-
Vol. 11
Abstract
Domestic pigs (Sus scrofa) share many genetic, anatomical, and physiological traits with humans and therefore constitute an excellent preclinical animal model. Fundamental understanding of the cellular and molecular processes governing early porcine cardiogenesis is critical for developing advanced porcine models used for the study of heart diseases and new regenerative therapies. Here, we provide a detailed characterization of porcine cardiogenesis based on fetal porcine hearts at various developmental stages and cardiac cells derived from porcine expanded pluripotent stem cells (pEPSCs), i.e., stem cells having the potential to give rise to both embryonic and extraembryonic tissue. We notably demonstrate for the first time that pEPSCs can differentiate into cardiovascular progenitor cells (CPCs), functional cardiomyocytes (CMs), epicardial cells and epicardial-derived cells (EPDCs) in vitro. Furthermore, we present an enhanced system for whole-embryo culture which allows continuous ex utero development of porcine post-implantation embryos from the cardiac crescent stage (ED14) up to the cardiac looping (ED17) stage. These new techniques provide a versatile platform for studying porcine cardiac development and disease modeling.
Keywords
- pig
- heart development
- porcine expanded pluripotent stem cells
- cardiac progenitor cells
- epicardial cells
- cardiomyocyte
