Investigation on the prevalence of chicken anaemia virus in poultry flocks of Kerala, India

Abstract

Chicken anaemia virus (CAV) is the aetiological agent of chicken infectious anaemia (CIA), an immunosuppressive disease, which brings a huge economic burden to the poultry industry globally. Concurrent infections and vaccination failures further aggravate the impact of the disease on the sector. The epidemiology of the disease and virulence of the circulating strains is to be known while formulating prevention strategies for any infectious disease. So far, there are no reports regarding the presence of CAV among poultry flocks in Kerala. In this scenario, the present study contemplated the investigation of the chicken anaemia virus using ELISA and PCR techniques. Indirect ELISA revealed the presence of CAV antibodies in 80 samples out of the 92 serum samples screened. Among the 100 samples collected from suspected cases, 29 were found to be positive for CAV infection with detection primers targeting the VP2 gene. This implies widespread distribution of the virus in Kerala.

Keywords

• chicken anaemia virus
• chicken infectious anaemia
• elisa
• pcr