PLoS Genetics (Apr 2017)

VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane.

  • David E Gordon,
  • Joanne Chia,
  • Kamburpola Jayawardena,
  • Robin Antrobus,
  • Frederic Bard,
  • Andrew A Peden

DOI
https://doi.org/10.1371/journal.pgen.1006698
Journal volume & issue
Vol. 13, no. 4
p. e1006698

Abstract

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The cellular machinery required for the fusion of constitutive secretory vesicles with the plasma membrane in metazoans remains poorly defined. To address this problem we have developed a powerful, quantitative assay for measuring secretion and used it in combination with combinatorial gene depletion studies in Drosophila cells. This has allowed us to identify at least three SNARE complexes mediating Golgi to PM transport (STX1, SNAP24/29 and Syb; STX1, SNAP24/29 and YKT6; STX4, SNAP24 and Syb). RNAi mediated depletion of YKT6 and VAMP3 in mammalian cells also blocks constitutive secretion suggesting that YKT6 has an evolutionarily conserved role in this process. The unexpected role of YKT6 in plasma membrane fusion may in part explain why RNAi and gene disruption studies have failed to produce the expected phenotypes in higher eukaryotes.