Genetic relationships in wild olives (Olea europaea ssp. oleaster) by ISSR and RAPD markers

Abstract

Wild olives or oleaster (Olea europaea ssp. oleaster) are found naturally in the Mediterranean basin. The changes in climate and environmental conditions have been gradually accelerating in the last decades. Since oleasters are more resistant to diseases, pests and unfavorable environmental conditions, they have become a very important source for resistance breeding studies that would be required in the future. Twelve inter simple sequence repeat (ISSR) and 12 random amplified polymorphic DNA (RAPD) markers were used; the effectiveness of these markers was studied and different parameters such as effective multiplex ratio, marker index, resolving power, and polymorphic information content, Shannon information index (I), effective allele number (ne), and Nei's genetic distance (h) were used in the determination of genetic varieties of 18 oleasters grown in Turkey. When both marker systems were analyzed in this study, it was observed that MI, RP and EMR parameters in ISSR gave higher values than RAPD while ne, I, and h were lower in ISSRs. Neighbour-joining dendrograms were developed by using Nei's genetic distance matrix, and principal coordinate analysis was performed.

Keywords

• Genetic variation
• ISSR
• RAPD
• wild olives