Nature Communications (Oct 2020)
Functional role of Tet-mediated RNA hydroxymethylcytosine in mouse ES cells and during differentiation
- Jie Lan,
- Nicholas Rajan,
- Martin Bizet,
- Audrey Penning,
- Nitesh K. Singh,
- Diana Guallar,
- Emilie Calonne,
- Andrea Li Greci,
- Elise Bonvin,
- Rachel Deplus,
- Phillip J. Hsu,
- Sigrid Nachtergaele,
- Chengjie Ma,
- Renhua Song,
- Alejandro Fuentes-Iglesias,
- Bouchra Hassabi,
- Pascale Putmans,
- Frédérique Mies,
- Gerben Menschaert,
- Justin J. L. Wong,
- Jianlong Wang,
- Miguel Fidalgo,
- Bifeng Yuan,
- François Fuks
Affiliations
- Jie Lan
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Nicholas Rajan
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Martin Bizet
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Audrey Penning
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Nitesh K. Singh
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Diana Guallar
- CiMUS, Universidade de Santiago de Compostela–Health Research Institute (IDIS)
- Emilie Calonne
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Andrea Li Greci
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Elise Bonvin
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Rachel Deplus
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Phillip J. Hsu
- Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, and Howard Hughes Medical Institute, University of Chicago
- Sigrid Nachtergaele
- Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, and Howard Hughes Medical Institute, University of Chicago
- Chengjie Ma
- Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University
- Renhua Song
- Epigenetics and RNA Biology Program Centenary Institute, The University of Sydney
- Alejandro Fuentes-Iglesias
- CiMUS, Universidade de Santiago de Compostela–Health Research Institute (IDIS)
- Bouchra Hassabi
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Pascale Putmans
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Frédérique Mies
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- Gerben Menschaert
- Department of Mathematical Modeling, Statistics and Bioinformatics, Faculty of Bioscience Engineering, Lab of Bioinformatics and Computational Genomics, Ghent University
- Justin J. L. Wong
- Epigenetics and RNA Biology Program Centenary Institute, The University of Sydney
- Jianlong Wang
- Department of Medicine, Columbia Center for Human Development (CCHD), Columbia University Irving Medical Center (CUIMC)
- Miguel Fidalgo
- CiMUS, Universidade de Santiago de Compostela–Health Research Institute (IDIS)
- Bifeng Yuan
- Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University
- François Fuks
- Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB Cancer Research Center (U-CRC), Welbio Investigator, Université Libre de Bruxelles (ULB)
- DOI
- https://doi.org/10.1038/s41467-020-18729-6
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 15
Abstract
TET mediated RNA-hydroxymethylation (5hmC) has been detected in mammals, but its physiological role remains unclear. Here the authors map 5hmC during embryonic stem cell (ESC) differentiation and find that Tet-mediated RNA hydroxymethylation reduces the stability of crucial pluripotency related transcripts.
