Stem Cell Research (May 2018)
Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa
- Yonglong Guo,
- Qiaolang Zeng,
- Shiwei Liu,
- Quan Yu,
- Peiyuan Wang,
- Hongjie Ma,
- Shanshan Shi,
- Xin Yan,
- Zekai Cui,
- Mengyuan Xie,
- Yunxia Xue,
- Qingbing Zha,
- Zhijie Li,
- Jun Zhang,
- Shibo Tang,
- Jiansu Chen
Affiliations
- Yonglong Guo
- Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China
- Qiaolang Zeng
- The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China
- Shiwei Liu
- The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China
- Quan Yu
- Centric Laboratory, Medical College, Jinan University, Guangzhou, China
- Peiyuan Wang
- The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China
- Hongjie Ma
- Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China
- Shanshan Shi
- Fetal Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, China
- Xin Yan
- Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China
- Zekai Cui
- Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China
- Mengyuan Xie
- Key Laboratory of Optoelectronic Information and Sensing Technologies, Guangdong Higher Educational Institutes, Jinan University, Guangzhou 510632, China
- Yunxia Xue
- Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou 510632, China; The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China
- Qingbing Zha
- Fetal Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, China
- Zhijie Li
- Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou 510632, China; The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China
- Jun Zhang
- Key Laboratory of Optoelectronic Information and Sensing Technologies, Guangdong Higher Educational Institutes, Jinan University, Guangzhou 510632, China
- Shibo Tang
- Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Correspondence to: S. Tang, Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China.
- Jiansu Chen
- Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou 510632, China; The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China; Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, China; Correspondence to: J. Chen, Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China.
- Journal volume & issue
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Vol. 29
pp. 139 – 142
Abstract
We have established an induced pluripotent stem (iPS) cell line using urine-derived cells from a 27-year-old male patient with retinitis pigmentosa associated with point mutations in the USH2A gene. Feeder-free culture conditions and the integration-free CytoTune™-iPS 2.0 Sendai Reprogramming Kit were used.
