Scientific Reports (Nov 2021)

Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin

  • Guan Wang,
  • Traci Kitaoka,
  • Ali Crawford,
  • Qian Mao,
  • Andrew Hesketh,
  • Fergus M. Guppy,
  • Garrett I. Ash,
  • Jason Liu,
  • Mark B. Gerstein,
  • Yannis P. Pitsiladis

DOI
https://doi.org/10.1038/s41598-021-00608-9
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 14

Abstract

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Abstract RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinant human erythropoietin for transcriptome-wide quantification of differential gene expression. The results show that total RNA DNB-seq generated a multitude of target genes compared to other platforms. Pathway enrichment analyses revealed genes correlate to not only erythropoiesis and oxygen transport but also a wide range of other functions, such as tissue protection and immune regulation. This study provides a knowledge base of genes relevant to EPO biology through cross-platform comparisons and validation.