PLoS ONE (Jan 2021)

Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence.

  • Xiaojun Tang,
  • Shisheng Li,
  • Xinming Yang,
  • Qinglai Tang,
  • Ying Zhang,
  • Shiying Zeng,
  • Mengmeng Li,
  • Kang Jiang,
  • Lu Guo,
  • Peiying Huang

DOI
https://doi.org/10.1371/journal.pone.0253943
Journal volume & issue
Vol. 16, no. 6
p. e0253943

Abstract

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ObjectiveTo screen for obstructive sleep apnea (OSA) biomarkers, isobaric tags for relative and absolute quantitation (iTRAQ)-labeled quantitative proteomics assay was used to identify differentially expressed proteins (DEPs) during chronic intermittent hypoxia (CIH).MethodThe iTRAQ technique was applied to compare DEPs in the serum of a CIH rat model and control group. Biological analysis of DEPs was performed using Gene Ontology and Kyoto Encyclopedia to explore related biological functions and signaling pathways. Enzyme-linked immunosorbent assay (ELISA) was performed to validate their expression in sera from patients with OSA and CIH rats.ResultsTwenty-three DEPs (fold change ≥1.2 or ≤0.833, p3 and higher coverage) were further verified by ELISA in the CIH rat model and OSA subject: apolipoprotein A-IV (APOA4, pConclusionAPOA4 and TUBA1A may be potential novel biomarkers for CIH and OSA, and may play an important role in the development of OSA complications.