Nature Communications (Aug 2017)

Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes

  • Sebastiaan J. Trietsch,
  • Elena Naumovska,
  • Dorota Kurek,
  • Meily C. Setyawati,
  • Marianne K. Vormann,
  • Karlijn J. Wilschut,
  • Henriëtte L. Lanz,
  • Arnaud Nicolas,
  • Chee Ping Ng,
  • Jos Joore,
  • Stefan Kustermann,
  • Adrian Roth,
  • Thomas Hankemeier,
  • Annie Moisan,
  • Paul Vulto

DOI
https://doi.org/10.1038/s41467-017-00259-3
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 8

Abstract

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Efforts to determine the effects of drugs on epithelial barriers could benefit from better in vitro models. Here the authors develop a microfluidic device supporting the growth and function of extracellular matrix-supported intestinal tubules, and evaluate the effect of staurosporine and acetylsalicylic acid on barrier integrity.