DNA self-assembly nanoflower reverse P-glycoprotein mediated drug resistance in chronic myelogenous leukemia therapy

Pengxuan Zhao; Yeteng Zhong; Pengcheng Pan; Shasha Zhang; Shasha Zhang; Yu Tian; Yu Tian; Jun Zhang; Guohui Yi; Zhendong Zhao; Tiantian Wu

doi:10.3389/fbioe.2023.1265199

Frontiers in Bioengineering and Biotechnology (Aug 2023)

DNA self-assembly nanoflower reverse P-glycoprotein mediated drug resistance in chronic myelogenous leukemia therapy

Pengxuan Zhao,
Yeteng Zhong,
Pengcheng Pan,
Shasha Zhang,
Shasha Zhang,
Yu Tian,
Yu Tian,
Jun Zhang,
Guohui Yi,
Zhendong Zhao,
Tiantian Wu

Affiliations

Pengxuan Zhao: Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China
Yeteng Zhong: Department of Clinical Laboratory, The Second Affiliated Hospital, Hainan Medical University, Haikou, China
Pengcheng Pan: Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China
Shasha Zhang: Wuhan Wuchang Hospital, Wuchang Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, China
Shasha Zhang: Public Research Center Hainan, Hainan Medical University, Haikou, China
Yu Tian: Analytical and Testing Center of Hainan University, Hainan University, Haikou, China
Yu Tian: Jiangsu Hengrui Pharmaceuticals Co., Ltd., Lianyungang, China
Jun Zhang: Department of Medical Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Guohui Yi: Public Research Center Hainan, Hainan Medical University, Haikou, China
Zhendong Zhao: Analytical and Testing Center of Hainan University, Hainan University, Haikou, China
Tiantian Wu: Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China

DOI: https://doi.org/10.3389/fbioe.2023.1265199
Journal volume & issue: Vol. 11

Abstract

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Introduction: Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder caused by the BCR-ABL chimeric tyrosine kinase. Vincristine (VCR) is widely used in leukemia therapy but is hindered by multidrug resistance (MDR).Methods: We prepared DNA nanoflower via self-assembly for the delivery of VCR and P-glycoprotein small interfering RNA (P-gp siRNA).Results and Discussion: The as-prepared nanoflower had a floriform shape with high loading efficiency of VCR (80%). Furthermore, the nanoflower could deliver VCR and P-gp siRNA into MDR CML cells and induce potent cytotoxicity both in vitro and in vivo, thus overcoming MDR of CML. Overall, this nanoflower is a promising tool for resistant CML therapy.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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