Molecular Regulation Mechanism of COPB2 Gene on Human Osteosarcoma Cell Based on Ingenuity Pathway Analysis

Abstract

Objective To explore the possible molecular regulation mechanism of COPB2 on human osteosarcoma cells based on the bioinformatics analysis of ingenuity pathway analysis (IPA). Methods RT-qPCR was used to detect the expression levels of COPB2 in human osteosarcoma cell lines U-2OS, MG-63, HOS and Saos-2. U-2OS was used as the research object and divided into silence group (KD) and control group (NC). U-2OS cells were infected by green fluorescent protein-labeled lentivirus and the KD group contained COPB2 silenced sequence. Human gene expression profiling microarray was used to detect the difference in gene expression profile of U-2OS cells after COPB2 silencing. The detection information was input into the IPA software for bioinformatics analysis, including classical pathway analysis, upstream regulation analysis, disease function analysis, regulatory effect analysis and interaction network analysis. Results COPB2 had a high expressive abundance in four human osteosarcoma cell lines. There were significant differences in the expression of 1393 genes between KD group and NC group; 831 genes were up-regulated and 562 genes were down-regulated. After COPB2 knockdown, the inhibition of angiogenesis by TSP1 pathway and acute phase response pathway were significantly activated, while cyclins and cell cycle regulation pathway were significantly inhibited. COPB2 mainly affected neoplasia, invasion and anoikis of osteosarcoma cells. Conclusion COPB2 is highly expressed in human osteosarcoma cell lines, and might be involved in the development of osteosarcoma through cyclins and cell cycle regulation pathway. In addition, COPB2 is associated with neoplasia, invasion and anoikis of osteosarcoma cells.

Keywords

• copb2
• osteosarcoma
• bioinformatics