Comparison of blood profiles between healthy and Brucella affected cattle

Abstract

Aim: The present investigation was undertaken to evaluate the blood biochemical metabolites of crossbred cattle suffering from brucellosis, in Khetri region of Kamrup District of Assam, India. Materials and Methods: Blood was collected by venipuncture from the jugular vein using 18 gauge hypodermic needle fitted to a Vacutainer-Leur adapter and evacuated tubes. Samples were screened for Brucella by rose Bengal plate test using plate test antigen. The study consisted of two groups. Group I consisted of healthy crossbred cows and Group II consisted of crossbred cows affected with brucellosis. Blood metabolites were estimated by spectrophotometric methods. Results: Out of 14 serum samples, 8 samples were positive for Brucella and remaining 6 were found to be negative. It was observed that serum albumin and urea of the affected cattle showed a significant decrease (p<0.05), but serum globulin, cholesterol, triglyceride, aspartate transaminase, and alanine transaminase activities were significantly increased (p<0.05) in comparison to the healthy cattle. Glucose, protein, albumin: globulin ratio, blood urea nitrogen did not show much variation between the Brucellosis affected and healthy cattle. Conclusion: Results obtained showed that brucellosis have a harmful effect on the animals health as they disturb the vital organs functions, which appear in the form of changes in blood biochemical parameters.

Keywords

• biochemical metabolites
• brucellosis
• cattle
• rose Bengal plate test