Retrieval and purification of human β cells from stem cell-derived islet engrafted mice

Jennifer Hyoje-Ryu Kenty; Gabriela Cristina Pelayo; Nayara Carvalho Leite

STAR Protocols (Sep 2021)

Retrieval and purification of human β cells from stem cell-derived islet engrafted mice

Jennifer Hyoje-Ryu Kenty,
Gabriela Cristina Pelayo,
Nayara Carvalho Leite

Affiliations

Jennifer Hyoje-Ryu Kenty: Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA; Corresponding author
Gabriela Cristina Pelayo: Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA
Nayara Carvalho Leite: Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 3
p. 100675

Abstract

Read online

Summary: Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a sufficient number of live β cells from mice following long-term human SC-β-cell engraftment. The protocol enables examination of SC-β-cells undergoing developmental and metabolic changes in vivo and may facilitate the understanding of metabolic demand on SC-β-cells.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords