Nature Communications (Nov 2021)
DHX15-independent roles for TFIP11 in U6 snRNA modification, U4/U6.U5 tri-snRNP assembly and pre-mRNA splicing fidelity
- Amandine Duchemin,
- Tina O’Grady,
- Sarah Hanache,
- Agnès Mereau,
- Marc Thiry,
- Ludivine Wacheul,
- Catherine Michaux,
- Eric Perpète,
- Eric Hervouet,
- Paul Peixoto,
- Felix G. M. Ernst,
- Yann Audic,
- Franck Dequiedt,
- Denis L. J. Lafontaine,
- Denis Mottet
Affiliations
- Amandine Duchemin
- GIGA-Molecular Biology of Diseases, Gene Expression and Cancer Laboratory, B34 +1, University of Liege
- Tina O’Grady
- GIGA-Molecular Biology of Diseases, Gene Expression and Cancer Laboratory, B34 +1, University of Liege
- Sarah Hanache
- GIGA-Molecular Biology of Diseases, Gene Expression and Cancer Laboratory, B34 +1, University of Liege
- Agnès Mereau
- CNRS, Institut de Génétique et Développement de Rennes (IGDR) UMR 6290, Université de Rennes
- Marc Thiry
- GIGA-Neurosciences, Unit of Cell Biology, B36 + 1, University of Liege
- Ludivine Wacheul
- RNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université Libre de Bruxelles (ULB) - Biopark campus Gosselies
- Catherine Michaux
- NARILIS, ILEE and NISM Institutes, Laboratory of Physical Chemistry of Biomolecules, University of Namur
- Eric Perpète
- NARILIS, ILEE and NISM Institutes, Laboratory of Physical Chemistry of Biomolecules, University of Namur
- Eric Hervouet
- INSERM UMR 1098 RIGHT, EPIgenetics and GENe EXPression Technical Platform (EPIGENExp), INSERM, EFS-BFC, University Bourgogne Franche-Comté
- Paul Peixoto
- INSERM UMR 1098 RIGHT, EPIgenetics and GENe EXPression Technical Platform (EPIGENExp), INSERM, EFS-BFC, University Bourgogne Franche-Comté
- Felix G. M. Ernst
- RNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université Libre de Bruxelles (ULB) - Biopark campus Gosselies
- Yann Audic
- CNRS, Institut de Génétique et Développement de Rennes (IGDR) UMR 6290, Université de Rennes
- Franck Dequiedt
- GIGA-Molecular Biology of Diseases, Gene Expression and Cancer Laboratory, B34 +1, University of Liege
- Denis L. J. Lafontaine
- RNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université Libre de Bruxelles (ULB) - Biopark campus Gosselies
- Denis Mottet
- GIGA-Molecular Biology of Diseases, Gene Expression and Cancer Laboratory, B34 +1, University of Liege
- DOI
- https://doi.org/10.1038/s41467-021-26932-2
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 20
Abstract
In yeast, TFIP11 and DHX15 promote the disassembly of spliceosome complex after splicing is completed. Here the authors show that human TFIP11 functions independently of DHX15 and is required for U6 snRNA 2’-O-methylation and U4/U6.U5 tri-snRNP assembly.
