Using the Box–Behnken response surface method to optimize the preparation and characterization of lavender and fennel volatile oil β-cyclodextrin form inclusion complex

Abstract

Objective: The objective of this study is to study the best inclusion technology of Lavender- and fennel-mixed volatile oil by beta-cyclodextrin (β-CD) and characterize the final product thereafter. Methods: Using the saturated water solution method, the volatile β-CD inclusion complex was produced. The effect of volatile oil weight ratio, inclusion temperature and inclusion time on the inclusive quality was studied by measuring the yield of inclusion and inclusion rate of volatile oil as evaluation indexes. The preparation method of inclusion complex was then optimized by the Box-Behnken response surface method. The inclusion complex was characterized by ultraviolet spectrophotometry, thin-layer chromatography, thermogravimetry and differential thermal analysis, and the microscopic imaging method. Results: The optimized conditions were the weight ratio of β-CD to volatile oil was 8.13:1 (g/ml). The inclusion temperature was 44°C. The inclusion time was 1 h. Conclusion: We were able to produce an inclusion complex with high inclusion rate of volatile oil and high yield of inclusion using the preparation method mentioned above. Furthermore, the method can also improve the stability of volatile oil in abnormal savda munziq. This study can provide a good reference for the development of new preparations.

Keywords

• Fennel
• inclusion complex
• lavender
• response surface method
• volatile oil
• β-cyclodextrin