Study of Codon 72 Polymorphism of TP53 Gene in Cervical Biopsies with Abnormal Histology in Bushehr Province

Abstract

Background: TP53 tumor suppressor gene play a role in repairing DNA damages and also in cell apoptosis. This research have paid to the association between codon 72 polymorphism of TP53 gene in a variety of abnormal cervical tissue samples compared to samples of healthy women as a control group and also frequency of human herpes simplex viruses (HSV) in these samples. Material and Methods: In this case-control study, 110 biopsies of the cervix with abnormal pathology were examined from archives of the Department of Pathology at Shohadaye Khalij Fars Hospital, and 164 healthy women were selected as control group. PCR test was used for detection of HSV, and Allele-specific PCR was used for analysis of codon 72 polymorphism of TP53 gene. Data was analyzed with SPSS software. Results: Distribution of codon 72 of TP53 genotypes in patient and control groups was not statistically significant. Evaluation of the frequency of arginine and proline alleles in patient and control groups revealed that those without proline allele were lower in patients (19%) compared to controls (30.1%), and this difference was statistically significant (p=0.04), (OR: 1.03-3.36، CI 95%=1.86). Also there were not significant correlation between the various results of pathology, age, ethnicity and place of residence with the TP53 genotype. In this study, HSV was not detected in samples. Conclusion: Because of significant difference in proline allele between the case and control groups, it seems that this allele is associated with the  abnormal cervical pathology results in Bushehr province.

Keywords

• Polymorphism
• TP53 gene
• Herpes simplex virus
• Allele-specific PCR