Purification, Characterization and Antioxidant Activities <em>in Vitro</em><em> </em>and <em>in Vivo</em> of the Polysaccharides from <em>Boletus edulis</em> Bull

Abstract

A water-soluble polysaccharide (BEBP) was extracted from <em>Boletus edulis </em>Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both <em>in vitro</em> and <em>in vivo </em>suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.

Keywords

• &lt;em&gt;Boletus edulis&lt;/em&gt; Bull
• polysaccharide
• purification
• antioxidant activity