Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele

Sho Yoshimatsu; Rei Murakami; Tsukika Sato; Tsubasa Saeki; Masafumi Yamamoto; Erika Sasaki; Toshiaki Noce; Hideyuki Okano

Stem Cell Research (May 2021)

Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele

Sho Yoshimatsu,
Rei Murakami,
Tsukika Sato,
Tsubasa Saeki,
Masafumi Yamamoto,
Erika Sasaki,
Toshiaki Noce,
Hideyuki Okano

Affiliations

Sho Yoshimatsu: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan
Rei Murakami: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Tsukika Sato: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Tsubasa Saeki: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
Masafumi Yamamoto: ICLAS Monitoring Center, Central Institute for Experimental Animals, Kanagawa, Japan
Erika Sasaki: Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Kanagawa, Japan
Toshiaki Noce: Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan
Hideyuki Okano: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Corresponding author.

Journal volume & issue: Vol. 53
p. 102308

Abstract

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POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA).

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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