PROTEOMIC MASS-SPECTROMETRIC ANALYSIS OF FRESHLY ISOLATED ESCHERICHIA COLI STRAINS

Abstract

Aim. Time-of-flight mass-spectrometric analysis of colon bacilli populations freshly isolated from humans for identification of proteome markers, that are characteristic for hemolytic and non-hemolytic strains, E.coli - associants of opportunistic bacteria. Materials and methods. 20 strains of monopopulation of hemolytic E.coli; 100 strains of monopopulation of non-hemolytic E.coli; 15 non-hemolytic strain, isolated from association with opportunistic bacteria. Protein profiling on MALDI-TOF MS Autoflex «Bruker Daltonik» mass-spectrometer. Results. Value within species for mass-charge peak with 100% intensity of 9000 Da could be a differential sign for isolation of hemolytic E.coli during mass-spectrometric biotyping. Conclusion. Proteomic characteristics of strains from various populations within E.coli are given. Taxon-specific markers for proteomic differentiation of E.coli populations are established.

Keywords

• escherichia coli
• mass-spectrometry
• mass-peak-list
• biotyping