Crystal structure of Drosophila Piwi

Sonomi Yamaguchi; Akira Oe; Kazumichi M. Nishida; Keitaro Yamashita; Asako Kajiya; Seiichi Hirano; Naoki Matsumoto; Naoshi Dohmae; Ryuichiro Ishitani; Kuniaki Saito; Haruhiko Siomi; Hiroshi Nishimasu; Mikiko C. Siomi; Osamu Nureki

doi:10.1038/s41467-020-14687-1

Nature Communications (Feb 2020)

Crystal structure of Drosophila Piwi

Sonomi Yamaguchi,
Akira Oe,
Kazumichi M. Nishida,
Keitaro Yamashita,
Asako Kajiya,
Seiichi Hirano,
Naoki Matsumoto,
Naoshi Dohmae,
Ryuichiro Ishitani,
Kuniaki Saito,
Haruhiko Siomi,
Hiroshi Nishimasu,
Mikiko C. Siomi,
Osamu Nureki

Affiliations

Sonomi Yamaguchi: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Akira Oe: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Kazumichi M. Nishida: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Keitaro Yamashita: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Asako Kajiya: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Seiichi Hirano: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Naoki Matsumoto: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Naoshi Dohmae: Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science
Ryuichiro Ishitani: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Kuniaki Saito: Invertebrate Genetics Laboratory, National Institute of Genetics
Haruhiko Siomi: Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku
Hiroshi Nishimasu: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Mikiko C. Siomi: Department of Biological Sciences, Graduate School of Science, The University of Tokyo
Osamu Nureki: Department of Biological Sciences, Graduate School of Science, The University of Tokyo

DOI: https://doi.org/10.1038/s41467-020-14687-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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PIWI-clade Argonautes bind PIWI-interacting RNAs (piRNAs) to silence transposons and maintain genome integrity in animal gonads. Here the authors present the crystal structure of a Drosophila Piwi–piRNA complex isolated from cultured fly ovarian somatic cells and find that it contains a non-canonical DVDK tetrad, and lacks slicer activity in vitro.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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