The P450-Monooxygenase Activity and CYP6D1 Expression in the Chlorfenapyr-Resistant Strain of <i>Musca domestica</i> L.

Kseniya Krestonoshina; Anastasia Melnichuk; Anna Kinareikina; Kseniya Maslakova; Liana Yangirova; Elena Silivanova

doi:10.3390/insects15060461

Insects (Jun 2024)

The P450-Monooxygenase Activity and CYP6D1 Expression in the Chlorfenapyr-Resistant Strain of <i>Musca domestica</i> L.

Kseniya Krestonoshina,
Anastasia Melnichuk,
Anna Kinareikina,
Kseniya Maslakova,
Liana Yangirova,
Elena Silivanova

Affiliations

Kseniya Krestonoshina: All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology—Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA)—Branch of Tyumen Scientific Centre SB RAS Institutskaya St. 2, Tyumen 625041, Russia
Anastasia Melnichuk: All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology—Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA)—Branch of Tyumen Scientific Centre SB RAS Institutskaya St. 2, Tyumen 625041, Russia
Anna Kinareikina: All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology—Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA)—Branch of Tyumen Scientific Centre SB RAS Institutskaya St. 2, Tyumen 625041, Russia
Kseniya Maslakova: All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology—Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA)—Branch of Tyumen Scientific Centre SB RAS Institutskaya St. 2, Tyumen 625041, Russia
Liana Yangirova: All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology—Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA)—Branch of Tyumen Scientific Centre SB RAS Institutskaya St. 2, Tyumen 625041, Russia
Elena Silivanova: All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology—Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA)—Branch of Tyumen Scientific Centre SB RAS Institutskaya St. 2, Tyumen 625041, Russia

DOI: https://doi.org/10.3390/insects15060461
Journal volume & issue: Vol. 15, no. 6
p. 461

Abstract

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The house fly Musca domestica L. is one of the most common insects of veterinary and medical importance worldwide; its ability to develop resistance to a large number of insecticides is well known. Many studies support the involvement of cytochrome P-450-dependent monooxygenases (P450) in the development of resistance to pyrethroids, neonicotinoids, carbamates, and organophosphates among insects. In this paper, the monooxygenase activity and expression level of CYP6D1 were studied for the first time in a chlorfenapyr-resistant strain of house fly. Our studies demonstrated that P450 activity in adults of the susceptible strain (Lab TY) and chlorfenapyr-resistant strain (ChlA) was 1.56–4.05-fold higher than that in larvae. In females of the Lab TY and ChlA strains, this activity was 1.53- and 1.57-fold higher, respectively (p p = 0.08) and 1.83 (p M. domestica is accompanied by an increase in P450-monooxygenase activity without changes in CYP6D1 expression.

Published in Insects

ISSN: 2075-4450 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science
Website: http://www.mdpi.com/journal/insects/

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