A Study on Hemostatic Properties of Polyphenols Crosslinked Gelatin Microspheres Based on Michael Addition Reaction

Abstract

Massive blood loss caused by severe injury is one of the important challenges in the present research of hemostatic materials. In this study, we first prepared polyphenols crosslinked gelatin through Michael addition reaction at 60 °C, and pH = 9.5 for 30 min, herein the mass ratio of polyphenols to gelatin was 1:50. Then emulsion crosslinking method of water-in-oil was used to obtain polyphenols crosslinked gelatin microspheres, including dopamine crosslinked gelatin microspheres (DOPA-GMs) and gallic acid crosslinked gelatin microspheres (GA-GMs). The results showed that the hemostatic microspheres with polyphenols crosslinked gelatin could effectively enhance the hemostatic properties of single-component gelatin microspheres (GMs), and this study provided a new method and strategy for rapid hemostatic microspheres.