Evaluation of the solubility of 11-keto-β-boswellic acid and its histological effect on the diabetic mice liver using a novel technique

Issa Al Amri; Fazal Mabood; Isam T. Kadim; Abdulaziz Alkindi; A. Al-Harrasi; Sulaiman Al-Hashmi; Ghulam Abbas; Ahmed Hamaed; Basant Ahmed; Jawaher Al-Shuhaimi; Samera Khalaf; Jamaluddin Shaikh

doi:10.14202/vetworld.2021.1797-1803

Veterinary World (Jul 2021)

Evaluation of the solubility of 11-keto-β-boswellic acid and its histological effect on the diabetic mice liver using a novel technique

Issa Al Amri,
Fazal Mabood,
Isam T. Kadim,
Abdulaziz Alkindi,
A. Al-Harrasi,
Sulaiman Al-Hashmi,
Ghulam Abbas,
Ahmed Hamaed,
Basant Ahmed,
Jawaher Al-Shuhaimi,
Samera Khalaf,
Jamaluddin Shaikh

Affiliations

Issa Al Amri: ORCiD; Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Fazal Mabood: ORCiD; Institute of Chemical Sciences, University of Swat KP, Pakistan.
Isam T. Kadim: ORCiD; Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Abdulaziz Alkindi: ORCiD; Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
A. Al-Harrasi: ORCiD; Natural and Medical Sciences Research Center, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Sulaiman Al-Hashmi: ORCiD; Natural and Medical Sciences Research Center, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Ghulam Abbas: ORCiD; Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Ahmed Hamaed: ORCiD; Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Basant Ahmed: ORCiD; Natural and Medical Sciences Research Center, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Jawaher Al-Shuhaimi: ORCiD; Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Samera Khalaf: ORCiD; Natural and Medical Sciences Research Center, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.
Jamaluddin Shaikh: ORCiD; School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, PO Box 33, PC 616, Birkat Al-Mouz, Nizwa, Sultanate of Oman.

DOI: https://doi.org/10.14202/vetworld.2021.1797-1803
Journal volume & issue: Vol. 14, no. 7
pp. 1797 – 1803

Abstract

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Background and Aim: The literature is scant on the effect of 11-keto-β-boswellic acid (KBA) on the liver of diabetes-induced mice. This study was designed to develop a rapid, sensitive, accurate, and inexpensive detection technique for evaluating the solubility of KBA obtained from the gum resin of Omani frankincense (Boswellia sacra) in the liver of streptozotocin-induced diabetic mice using Fourier transform infrared (FTIR) reflectance spectroscopy coupled with principal components analysis (PCA). It also aimed to investigate the effect of KBA on histological changes in the hepatocytes of diabetic mice. Materials and Methods: Eighteen mice were assigned to the healthy control group, the diabetic control group, or the KBA-treated diabetic group. Liver tissue samples from all groups were scanned using an FTIR reflectance spectrophotometer in reflection mode. FTIR reflectance spectra were collected in the wavenumber range of 400-4000 cm-1 using an attenuated total reflectance apparatus. Results: FTIR reflectance spectra were analyzed using PCA. The PCA score plot, which is an exploratory multivariate data set, revealed complete segregation among the three groups' liver samples based on changes in the variation of wavenumber position in the FTIR reflectance spectra, which indicated a clear effect of KBA solubility on treatments. Histological analysis showed an improvement in the liver tissues, with normal structures of hepatocytes exhibiting mild vacuolation in their cytoplasm. Conclusion: KBA improved the morphology of liver tissues in the diabetic mice and led to complete recovery of the damage observed in the diabetic control group. FTIR reflectance spectroscopy coupled with PCA could be deployed as a rapid, low-cost, and non-destructive detection method for evaluating treatment effects in diseased liver tissue based on the solubility of KBA.

Published in Veterinary World

ISSN: 0972-8988 (Print); 2231-0916 (Online)
Publisher: Veterinary World
Country of publisher: India
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: http://www.veterinaryworld.org

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