Acta Biomedica Scientifica (May 2013)
COMPARATIVE EVALUATION OF CYTOKINE PROFILE IN EXPERIMENTAL ANIMALS UNDER THE EFFECT OF PHYSICAL FACTORS (PRELIMINARY STUDY)
Abstract
The results of studies of cytokine production in the serum of experimental animals exposed by general vibration and noise of industrial frequency. The aim of the work was comparative research of pro- and anti-inflammatory cytokines in animals under the effect of physical factors. 2 experiments of 4 series of studies within 15, 30, 60 and 120 days were conducted. Intact male albino rats were used as a control group. In all animals serum levels of cytokines IL-1β, IL-10, TNF-a (species-specific diagnostic kits Bender MedSystems (Austria)) were evaluated by ELISA. Mathematical processing of the results was carried out by methods of nonparametric statistics. Experiments on outbred albino rats determined both general patterns and features of the cytokine balance changes depending on the nature of the influence of physical factors in the dynamics of development of disorders in immune system. The decrease of IL-10 level under the influence of vibration is observed in 15 days and the one under the influence of noise is observed in 60 days. The decrease of IL-1β in rats is typical only at the effects of noise in 30 days and doesn't change at the effect of vibration. It was concluded that changes in the contents of cytokines in experimental animals under the effect of general vibration and noise production rate shows different mechanisms basic for the regulation of immune response.
