OsAMT1.1 knockout-induced decrease in cadmium absorption and accumulation by rice related to cadmium absorption-related gene downregulation

Abstract

Cadmium (Cd) is a hazardous heavy metal that poses a serious risk to human health through the food chain, with rice being a significant vector because of its tendency to accumulate Cd. Nitrogen (N), an essential element for plant growth, also affects the Cd absorption and accumulation in crops. This study investigated the effects of N application on Cd absorption and accumulation in Cd-contaminated soils. Potting experiment showed that increasing N concentrations significantly increased the plant biomass and Cd contents in rice tissues. Ammonium (NH4+) transporter gene OsAMT1.1 knockout led to a substantial reduction in Cd absorption and accumulation in all rice tissues compared to that in the wild-type plants. Specifically, osamt1.1 mutants increased the Cd content in culm tissues, whereas it was reduced in brown rice. In addition, OsAMT1.1 knockout reduced Cd2+ influx in roots under NH4+-N addition, although OsAMT1.1 lacked Cd transport ability when expressed in yeast. Gene expression analysis revealed that OsAMT1.1 knockout reduced Cd absorption-related genes (OsIRT1, OsNRAMP1, and OsNRAMP5) expression levels. These finding highlight the critical role of N supply and OsAMT1.1 in regulating the Cd content in rice, offering insights into the molecular mechanisms of Cd transportation in plants.

Keywords

• Cd absorption
• Cd accumulation
• Nitrogen
• OsAMT1.1
• Gene expression
• Rice