In Vitro Analysis of the Immunomodulating Effects of Allium Hookeri on Lymphocytes, Macrophages, and Tumour Cells

Abstract

The present study investigated the effects of ethanol extracts of Allium hookeri (leaf, root, and fermented root) on parameters of innate immunity, tumour cell viability and antioxidant effect in vitro. Innate immunity was measured by spleen lymphocyte proliferation, nitric oxide production by chicken macrophage HD11 cells and suppressive effect on tumour cell viability was assessed using chicken RP9 cells. Free radical scavenging capacity as a measure of antioxidant capacity was determined by 0.15 mM of DPPH solution. In vitro culture of chicken spleen lymphocytes with ethanol extract of Allium hookeri (62.5-500 μg/mL) significantly induced higher proliferation compared with media control. Stimulation of macrophages with ethanol extract of Allium hookeri (62.5-500 μg/mL) showed increased Nitric oxide production. Tumor cells growth was significantly inhibited by extracts of Allium hookeri at 15.6-125 μg/mL compared with medium control and all extracts exhibited greater than 80% scavenging activity at 1000 μg/mL compared with ethanol vehicle control. Above all, fermented root extracts showed strongest effects on antioxidant activity compared to leaf and root extracts.

Keywords

• allium hookeri
• innate immunity
• lymphocytes
• macrophage
• poultry
• tumour cells