Structural insights into the mechanism of rhodopsin phosphodiesterase

Tatsuya Ikuta; Wataru Shihoya; Masahiro Sugiura; Kazuho Yoshida; Masahito Watari; Takaya Tokano; Keitaro Yamashita; Kota Katayama; Satoshi P. Tsunoda; Takayuki Uchihashi; Hideki Kandori; Osamu Nureki

doi:10.1038/s41467-020-19376-7

Nature Communications (Nov 2020)

Structural insights into the mechanism of rhodopsin phosphodiesterase

Tatsuya Ikuta,
Wataru Shihoya,
Masahiro Sugiura,
Kazuho Yoshida,
Masahito Watari,
Takaya Tokano,
Keitaro Yamashita,
Kota Katayama,
Satoshi P. Tsunoda,
Takayuki Uchihashi,
Hideki Kandori,
Osamu Nureki

Affiliations

Tatsuya Ikuta: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo
Wataru Shihoya: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo
Masahiro Sugiura: Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-Ku
Kazuho Yoshida: Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-Ku
Masahito Watari: Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-Ku
Takaya Tokano: Department of Physics, Nagoya University
Keitaro Yamashita: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo
Kota Katayama: Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-Ku
Satoshi P. Tsunoda: Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-Ku
Takayuki Uchihashi: Department of Physics, Nagoya University
Hideki Kandori: Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-Ku
Osamu Nureki: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo

DOI: https://doi.org/10.1038/s41467-020-19376-7
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 12

Abstract

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Rhodopsin phosphodiesterase (Rh-PDE) hydrolyzes both cAMP and cGMP in a light-dependent manner. Structural and functional analyses of the Rh-PDE from Salpingoeca rosetta reveal unusual rhodopsin topology comprising 8 transmembrane helices (TMs) and suggest that TM0 plays a crucial role in the enzymatic photoactivity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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