Медицинская иммунология (Aug 2019)

CONTENT OF Th17-RELATED AND Th2 CYTOKINES IN ASTHMA PATIENTS WITH COLD AIRWAY HYPERRESPONSIVENESS

  • Aleksey B. Pirogov,
  • Anna G. Prikhodko,
  • Natalia A. Pirogova,
  • Denis E. Naumov,
  • Dina A. Gassan,
  • Juliy M. Perelman

DOI
https://doi.org/10.15789/1563-0625-COT-3020
Journal volume & issue
Vol. 0, no. 0

Abstract

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Summary. The phenomenon of cold airway hyperresponsiveness is very common among patients with bronchial asthma. Possible participation of immune mechanisms in its formation is insufficiently studied. In particular, there is no information about the interaction of Th17-related cytokines with cytokines of Th2 immune response accompanying inflammation in asthma patients with cold-induced bronchospasm. Objective. To evaluate the content of interleukins IL-17A, IL-6, IL-22, IL-4 and IL-13 in asthma patients and their role in the formation of cold airway hyperresponsiveness. Materials and Methods. Spirometric indices of forced expiratory flow were measured in 41 patients with bronchial asthma, the content of interleukins in blood serum was estimated before and after bronchoprovocation test with 3-minute isocapnic hyperventilation with cold (-20°C) air. Results. Two groups of patients were formed with presence (group 1, n=14) and absence (group 2, n=29) of cold airway hyperresponsiveness, verified by the degree of drop in forced expiratory volume in 1 sec. (∆FFV1ihca) after the cold test (-16.5[-20.0;-12.0] and -2.3[-3.5;-0.8] %, respectively; p<0.0001). In group 1 in relation to group 2, lower baseline values of FEV1 (88,1±3,1 and 96,6±2,2%, p=0,044) and forced midexpiratory flow (MEF25-75 62,4±3,87 and 75,6±3,7%, p=0,013) were registered. In addition, the baseline content of IL-17A, IL-6, IL-4 in subjects with cold airway hyperresponsiveness was significantly higher than in patients who did not respond to cold air. There was a correlation between IL-17A content in blood and severity of bronchial reaction (∆FEV1ihca) to cold test (Rs=-0,33; p=0,049). Conclusions. In asthma patients with cold airway hyperresponsiveness, high content of IL-17A, IL-6 and IL-4 indicates the participation of both Th2 and Th1/Th17 cytokines in the regulation of cold-induced bronchospasm and immune response with the formation of immune inflammation of “Th2 low” subtype.

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