Frontiers in Genetics (Mar 2022)

Genetic Variants of MicroRNA and DROSHA Genes in Association With the Risk of Tuberculosis in the Amazon Population

  • Diana Feio da Veiga Borges Leal,
  • Mayara Natália Santana da Silva,
  • Lucas Favacho Pastana,
  • Marianne Rodrigues Fernandes,
  • Aidalucy do Socorro Costa de Athayde,
  • Débora Christina Ricardo Fernandes Porchera,
  • Cleonardo Augusto da Silva,
  • Antônio André Conde Modesto,
  • Paulo Pimentel De Assumpcão,
  • Sidney Emanuel Batista dos Santos,
  • Sidney Emanuel Batista dos Santos,
  • Ney Pereira Carneiro dos Santos

DOI
https://doi.org/10.3389/fgene.2022.850058
Journal volume & issue
Vol. 13

Abstract

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Tuberculosis (TB) is a chronic infection caused by Mycobacterium tuberculosis (Mtb) with high incidence and mortality. Studies reported that host genetic variants might be associated with the risk of tuberculosis. The aim of this study was to perform an association study between 26 single nucleotide polymorphisms (SNPs) and tuberculosis and evaluate whether these SNPs may confer risk factors to tuberculosis in the Amazon population. There were 52 males and 126 females, with total of 178 healthy controls. Genotyping was performed using TaqMan Open Array Genotyping. Ancestry-informative markers were used to estimate the ancestral proportions of the individuals in the case and control groups. The results indicated that the SNPs rs10035440 (DROSHA), rs7372209 (miR26-a1), rs1834306 (miR100), rs4919510 (miR608), and rs10739971 (pri-let-7a-1) were significantly associated with high risk and rs3746444 (miR499) and rs6505162 (miR423), with low risk of developing tuberculosis in the Amazon population. Our study concluded that seven miRNA polymorphisms were associated with tuberculosis. Our study contributes to a better understanding of TB pathogenesis and may promote the development of new diagnostic tools against M. tuberculosis infection.

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