Study on anti-tumor activity of itraconazole in colon cancer

Abstract

Itraconazole is a common antifungal drug, which inhibiting lanosterol 14 alpha-demethylase, interfering with lanosterol convert to ergosterol or cholesterol, thereby affecting the synthesis of fungal cell membranes. In recent years, it has been found that it has anti-cancer effect and is expected to be used to treat a variety of human cancers. Colon cancer is a cancer disease that affects the health of contemporary people and its incidence tends to be younger. In this paper, the effects of itraconazole on proliferation and migration of Caco2 and HT-29 of two kinds of colon cancer cells were detected by MTT, scratch experiment and western blot. The data showed that itraconazole concomitant with increased drug concentration caused cell morphology of colon cancer significantly shrink. MTT experiment showed that it could reduce the survival rate of colon cells and western blot data indicated that it could down-regulation PCNA and MYL9 which are marker genes of proliferation and migration. Furthermore, the cell migration is reduced.