Біологічні студії (Sep 2015)
Amino acids and polyamines content in pectoral muscle of broiler chickens under using spleen extract for prevention of pre-slaughter stress
Abstract
The results on the Adrenocorticotropic hormone (ACTH) level in blood, amino acids and polyamines content in pectoral muscle of broiler chickens under using spleen extract for decreasing pre-slaughter stress are presented. The spleen extract (70% alcohol solution in volume 1.4 ml per chicken) was added to the diet of broiler chickens of experimental groups by using aerosol method. This extract was obtained with and without the ultrasound application. 70% alcohol solution in the same volume and the same method was used to the diet of broiler chickens of control group five days before slaughter. The content of polyamines, such as putrescin, spermine and spermidin in pectoral muscle of broiler chickens was determined. The total amount of polyamines and spermine specifically of both experimental groups in broiler chickens pectoral muscle was reliable higher compared to the control. The level of putrescin, spermine and spermidin was the lowest in pectoral muscle of broiler chickens of control group. The putrescin content in the pectoral muscle was reliablely highest in broiler chickens of the first experimental group after addition to the diet of the spleen extract with the ultrasound application. The concentration of amino acids, except of serine, in broiler chickens pectoral muscle was reliablely highest in broiler chickens of both experimental group, compared to control. The amount of polyamines and spermine specifically of both experimental groups in pectoral muscle of broiler chickens was reliablely higher, compared to control. After using of spleen extract, obtained with and without the ultrasound application, ACTH level in blood of broiler chickens was reliablely lower compared to control, that caused a decrease at the pre-slaughter stress. The amino acids content in pectoral muscle of broiler chickens was reliablely the highest in broiler chickens of both experimental group after addition of the spleen extract to the diet that caused a raise in biological quality of poultry meat.
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