MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells

Iris Müller; Ann Sophie Moroni; Daria Shlyueva; Sudeep Sahadevan; Erwin M. Schoof; Aliaksandra Radzisheuskaya; Jonas W. Højfeldt; Tülin Tatar; Richard P. Koche; Chang Huang; Kristian Helin

doi:10.1038/s41467-021-23308-4

Nature Communications (May 2021)

MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells

Iris Müller,
Ann Sophie Moroni,
Daria Shlyueva,
Sudeep Sahadevan,
Erwin M. Schoof,
Aliaksandra Radzisheuskaya,
Jonas W. Højfeldt,
Tülin Tatar,
Richard P. Koche,
Chang Huang,
Kristian Helin

Affiliations

Iris Müller: Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen
Ann Sophie Moroni: Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen
Daria Shlyueva: Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen
Sudeep Sahadevan: Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen
Erwin M. Schoof: The Finsen Laboratory, Rigshospitalet and Biotech Research and Innovation Centre (BRIC), Faculty of Health Sciences, University of Copenhagen
Aliaksandra Radzisheuskaya: Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen
Jonas W. Højfeldt: Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen
Tülin Tatar: Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen
Richard P. Koche: Center for Epigenetics Research, Memorial Sloan Kettering Cancer Center
Chang Huang: Cell Biology Program and Center for Epigenetics Research, Memorial Sloan Kettering Cancer Center
Kristian Helin: Biotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-021-23308-4
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 17

Abstract

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Naïve pluripotency is characterized by distinctly open chromatin and repressed endogenous retroviruses. Here the authors show that MPP8 and its association with the core HUSH complex is essential for naïve pluripotent cells; also that repression of LINE1 elements by MPP8 does not require chromatin binding, nor H3K9me3.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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